周南南

目的：本文旨在研究死盒解旋酶33（DHX33）在调控肺癌细胞迁移和凋亡中的作用。方法：利用shRNA技术敲低肺癌细胞NCI-H2228中的DHX33，并通过实时荧光定量PCR和Western blotting验证DHX33的敲低效率；通过细胞划痕和流式细胞术检测DHX33敲低后对肺癌细胞迁移和凋亡的影响。结果：利用shRNA技术在mRNA水平（P<0.0001）和蛋白水平均显著抑制了肺癌细胞中DHX33的表达量。与对照组肺癌细胞相比，DHX33的敲低显著降低了肺癌细胞的迁移能力（P<0.05），并且还显著促进肺癌细胞的凋亡水平（P<0.05）。结论：敲低DHX33可抑制肺癌细胞NCI-H2228的迁移并促进其凋亡。

肺癌细胞；DHX33；基因敲低；细胞迁移；细胞凋亡

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