孔 伟,任小桐,柳赟昊

目的：探究自噬在氯化镉（CdCl2）诱导小鼠睾丸间质细胞凋亡中的作用机制。方法：不同浓度CdCl2（0、5和10μmol/L）染毒细胞24h，Western blot检测细胞内凋亡蛋白cleaved Caspase-3和cleaved Caspase-9以及自噬相关蛋LC3、p62蛋白表达水平。分别用自噬抑制剂3-甲基腺嘌呤（3-Methyladenine，3MA）（60µmol/L）和自噬激动剂雷帕霉素（Rapamycin，RAP）（50nmol/L）与CdCl2（10μmol/L）染毒TM3细胞24h，Western blot检测细胞内LC3、p62、cleaved Caspase-3和cleaved Caspase-9蛋白表达水平的变化。结果：与对照组细胞相比，随着CdCl2浓度增加，cleaved Caspase-9, cleaved Caspase-3、LC3-II和p62蛋白表达水平显著升高（P < 0.05）。与CdCl2组相比，CdCl2与3-MA共处理组细胞中LC3-II、p62、cleaved Caspase-9和cleaved Caspase-3蛋白的表达显著下调（P<0.001），CdCl2与RAP共处理组细胞中四种蛋白的表达水平也均显著降低（P<0.001）。结论：氯化镉可能通过阻滞自噬流，促使自噬体异常聚集，引起睾丸间质细胞凋亡发生。

镉；睾丸间质细胞；自噬体；凋亡

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