魏宇航

基因编辑技术具有精确编辑基因组上目标基因的能力。在多种基因编辑工具中，CRISPR/Cas9系统是一种革命性的技术，它具有结构简单、特异性强、切割效率高等优势，已被广泛应用于各个领域，取得显著的成果。但脱靶效应的存在一直阻遏该技术的应用与发展。该文主要对CRISPR/Cas9的产生机制进行简述，并根据脱靶机制开发的降低策略进行总结。

CRISPR/Cas9系统；脱靶效应；合理工程设计

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